Catch Bond Interaction between Cell-Surface Sulfatase Sulf1 and Glycosaminoglycans
نویسندگان
چکیده
منابع مشابه
Catch bond interaction between cell-surface sulfatase Sulf1 and glycosaminoglycans.
In biological adhesion, the biophysical mechanism of specific biomolecular interaction can be divided in slip and catch bonds, respectively. Conceptually, slip bonds exhibit a reduced bond lifetime under increased external force and catch bonds, in contrast, exhibit an increased lifetime (for a certain force interval). Since 2003, a handful of biological systems have been identified to display ...
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BACKGROUND Sulf1 is a cell-surface sulfatase removing internal 6-O-sulfate groups from heparan sulfate (HS) chains. Thereby it modulates the activity of HS-dependent growth factors. For HS interaction Sulf1 employs a unique hydrophilic domain (HD). METHODS Affinity-chromatography, AFM-single-molecule force spectroscopy (SMFS) and immunofluorescence on living cells were used to analyze specifi...
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We used ELISA and flow cytometry to study the binding of prion protein PrP to glycosaminoglycans (GAGs). We found that recombinant human PrP (rPrP) binds GAGs including chondroitin sulphate A, chondroitin sulphate B, hyaluronic acid, and heparin. rPrP binding to GAGs occurs via the N-terminus, a region known to bind divalent cations. Additionally, rPrP binding to GAGs is enhanced in the presenc...
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Extracellular sulfatases (SULF1; SULF2) (EC: 3.1.6.-) are members of the sulfatase enzyme family which exhibit endoglucosamine-6-sulfatase activity and carry out essential roles in proteoglycan metabolism. These enzymes regulate a number of critical signalling pathways and the sulfation state of glycoaminoglycans in the extracellular space. SULF1 and SULF2 amino acid sequences and structures an...
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The interaction between human plasma lipoproteins and glycosaminoglycans has been studied by use of gels consisting of cross-linked hyaluronic acid and of sulfated glycosaminoglycans covalently attached to agarose (Sepharose 4B). When chromatographed on a column of granulated hyaluronic acid gel at physiological pH and ionic strength, very low density (VLDL), low density (LDL), and high density...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2015
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2015.02.028